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ExpiCHO™ Expression Medium

日期: 2015-11-16
浏览次数: 202

货号:A2910002

 规格

Form:     Liquid

Format:  Bottle(s)

Cell Line:   CHO-S, SFM Adapted

Product Size:   6 x 1 L

Classification:   Animal Origin-Free, Protein-Free, Chemically Defined, Serum-Free

Shipping Condition:   Room Temperature

内容及储存

Storage conditions: in the dark at 2-8°C

Shipping conditions: ambient

Shelf life: 12 months from date of manufacture

描述

 ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:
• Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
• Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
• Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
• Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
• Supports growth and transfection of CHO cells in culture formats from <1 ml="" in="" multi-well="" plates="" to="">10 L in benchtop bioreactors
• Does not contain phenol red

ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 ml="" in="" multi-well="" plates="" to="">10 L in bioreactors.






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