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GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit
货号: A21175  规格  Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ⁄ MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion  内容及储存  Contains:• CRISPR CD4 Nuclease Vector, linearized• 10X Oligonucleotide Annealing Buffer• DNase/RNase-free Water• 5X Ligation Buffer• T4 DNA Ligase• U6 Forward Sequencing Primer• Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C).  描述  The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...
2015 - 06 - 01
GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit
货号: A21175  规格  Promoter: CMV, U6 Reporter: CD4 Key Function: Gene Editing, Stable Cell Line Development, Genome Engineering, Episomal Expression Product Size: 10 reactions Cloning Method: Restriction Enzyme ⁄ MCS Delivery Method: Transfection Protein Tag or Fusion: CD4 fusion  内容及储存  Contains:• CRISPR CD4 Nuclease Vector, linearized• 10X Oligonucleotide Annealing Buffer• DNase/RNase-free Water• 5X Ligation Buffer• T4 DNA Ligase• U6 Forward Sequencing Primer• Control Double-stranded OligonucleotideStore in freezer (-5 to -30°C).  描述  The GeneArt® CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads® CD4 magnetic beads. Transfection ...
2015 - 06 - 01
TRIzol® Reagent
货号: 15596018  规格  Sample Type (General): Bacteria, Yeast, Blood, Plant Samples, Tissue, Cells, Viral Samples High Throughput Compatibility: Not High Throughput-Compatible (Manual) Downstream Application: Reverse Transcriptase PCR (RT-PCR), Cloning, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, cDNA Library Construction, Northern Blotting Scale: Scaleable System: TRIzol Quantity: 1 Product Size: 200 mL Final Product: Total RNA, micro RNA, Transcriptome RNA Purification Time: 1 hr Number of Reactions: 200 Preps Isolation Technology: Organic Extraction Starting Material (Amount): Up to 1 x 10^7 cells, Up to 1 g tissue Shipping Condition: Room Temperature  内容及储存  包括TRIzol® 试剂。置于2°C至8°C保存。  描述  TRIzol® 试剂是一种完全的即用型试剂,可用于提取高质量的总RNA或者从各种生物样本中同时提取RNA、DNA和蛋白质。这种酚和异硫氰酸胍单相溶液,可用于提取人、动物、植物、酵母或细菌来源的细胞和组织样本中的纯RNA、DNA和蛋白质,只需一个小时即可完成。• 从一份样品同时提取RNA、DNA和蛋白质• 即便是难以处...
2015 - 05 - 27
Lipofectamine® 2000 Transfection Reagent
货号: 11668019  规格  Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells Sample Type (Specific): Plasmid DNA, RNAi Plasmids (shRNA, miR), Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes Product Size: 1.5 mL Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice High Throughput Compatibility: High Throughput-Compatible Green Features: Sustainable packaging  内容及储存  置于+4°C保存。Lipofectamine™ 2000试剂正确储存时可稳定保存6个月。  描述  Lipofectamine® 2000转染试剂是一种多用途转染试剂,可在各种贴壁和悬浮细胞系中提供高效转染。研究人员可使用Lipofectamine® 2000试剂进行基于siRNA和shRNA的基因敲除实验及基因表达研究。Lipofectamine® 2000是Life Technologies提供的用于siRNA和质粒DNA共转染的最佳试剂。采用Lipofectamine® 2000试剂,您可以实现:• 在各种细胞系中具有卓越的转染效率和高重组蛋白表达水平 (表1)• 出众的siRNA和质粒DNA共转染性能• 在血清存在时亦具有可靠的转染效率 – 转染后无需更换培养基• 适用于高通量应用的可靠性能• 建立稳定细胞系的最佳选择适用于基因表达和基因沉默的高性能转染试剂Lipofectamine® 2000试剂适合所有常见细胞系及许多难以转染的细胞系,可用于含有或不含血清的培养基。在基因沉默应用中...
2015 - 05 - 27
PURELINK RNA MINI KIT
货号: 12183020 规格 Sample Type (General):Bacteria, Yeast, Blood, Tissue, Plant Samples, CellsHigh Throughput Compatibility:Not High Throughput-Compatible (Manual)Downstream Application:Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR)Scale:MiniSystem:PureLink™Quantity:10 columnsProduct Size:10 prepsFinal Product:Total RNAYield (up to):1000 µgNumber of Reactions:10 PrepsElution Volume - max:300 µlElution Volume - min:30 µlIsolation Technology:Silica Spin ColumnStarting Material (Amount):Up to 200 mg tissue, 5 x 10^6 to 1 x 10^8 cellsShipping Condition:Room Temperature内容及储存 The PureLink® RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components sho...
2015 - 05 - 25
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北京优健萌威医药科技有限公司是代理多品牌的试剂和耗材。自2012年公司成立以来,我们秉承追求卓越品质,提供一流服务的理念,以客户利益最大化,从客户角度出发,主张建立双赢体系, 目前我们的合作伙伴已超过2000余家。作为生命科学领域的专业服务商, 我们将为您提供优质、创新的生命科学解决方案,完善、贴心的客户服务体验,尽可能满足您所有的实验需求。      期待与您一起携手,探寻生命科学之美!
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