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ExpiCHO™ Expression Medium

日期: 2015-11-13
浏览次数: 280

货号: A2910001

规格

Form:  Liquid

Format:  Bottle(s)

Cell Line:  CHO-S, SFM Adapted

Product Size:    1000 mL

Classification:  Animal Origin-Free, Protein-Free, Chemically Defined, Serum-Free

Shipping Condition: Room Temperature


内容及储存

Storage conditions: in the dark at 2-8°C

Shipping conditions: ambient

Shelf life: 12 months from date of manufacture


描述

     ExpiCHO™ Expression Medium is a   GMP-manufactured, chemically-defined, serum-free, protein-free, animal  origin–free medium specifically designed for high-density growth and  transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a  core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) forscalable transient protein production.

ExpiCHO Expression Medium:

Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability

Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with
   ExpiFectamine™ CHO transfection reagent

Supports transient transfection of suspension CHO cells at a  density of 6 x 106 cells per mL

Facilitates sustained, high-level expression of high-density  transiently transfected cultures, achieving yields of up to 3 g per L of
 recombinant protein

Supports growth and transfection of CHO cells in culture formats  from <1 ml="" in="" multi-well="" plates="" to="">10 L in benchtop bioreactors

Does not contain phenol red

     ExpiCHO Expression Medium is formulated    with GlutaMAX™-I reagent. It is ready to use, with no supplementation required.The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which  is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell  cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker  platform in a humidified, CO2 cell culture incubator. However, cultures are  scalable from volumes of <1 ml="" in="" multi-well="" plates="" to="">10 L in bioreactors.





 

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