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Essential 6™ Medium

日期: 2015-06-01
浏览次数: 449

货号: A1516401


规格

 

Cell Type: Stem Cells (iPS - Induced Pluripotent Stem)
Form: Liquid
Species: Human
Quantity: 1 bottle(s)
Serum Level: Serum-Free
Culture Type: Feeder Cell-Free
Product Size: 500 mL
Classification: Xeno-Free
Volume (Metric): 500 ml

 

内容及储存

 

1 × 500 ml bottle, Store at 2 to 8°C. Protect from light.

 

描述

 

Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6™ Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus). In addition, Essential 6™ Medium can be used as a base for the formulation of custom media for the culture of PSCs.

Essential 6™ Medium enables: 

 Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
 Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
 Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application

Reprogramming
Essential 6™ Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune™ (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability. 

Differentiation 
Essential 6™ Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6™ Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation. 

Flexibility
Essential 6™ Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application. 













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