内容及储存
6 × 2.5 mL dropper bottles
Store at ≤ 25°C.
描述
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue® Fixed Cell ReadyProbes® Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.• No need to dilute, weigh, or pipette• Convenient dropper bottle—just use two drops per mL• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filterSee other ReadyProbes® reagents for cell stainingSee other nuclear stains for imagingCell imaging applications DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..Suggestions for useIn most cases 2 drops/ml of NucBlue® Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.• NucBlue® Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications.