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货号: 10777019  规格  Product Size: 5,000 units Shipping Condition: Dry Ice  描述  RNaseOUT™ 重组核糖核酸酶抑制剂 (RNaseOUT™ Recombinant Ribonuclease Inhibitor) 是胰型核糖核酸酶例如 RNase A 的强效非竞争性抑制剂。如同其它 RNase 抑制剂,RNaseOUT™ 重组核糖核酸酶抑制剂是酸性蛋白质,分子量约为 52 kDa。 RNaseOUT™ 抑制 RNase A、RNase B 和 RNase C。应用:cDNA 合成、RT-PCR (2),以及体外转录和翻译。来源: 通过亲和色谱从表达克隆的猪基因的大肠杆菌 (E. coli ) 中纯化。性能和质量测试: SDS-PAGE 纯度、内切脱氧核糖核酸酶测定、蛋白质浓度、比活;经过 RT-PCR 性能评估。单位定义: 使用胞苷 2´, 3´ 环单磷酸 (cCMP) 作为底物,一个单位可对 5 ng 的 RNase A 产生 50% 的抑制效果 (1)。单位反应条件: 反应物共 1 ml,包含 100 mM Tris-醋酸 (pH 6.5)、1 mM EDTA、0.2 mM cCMP、2 µg RNase A,反应时间 0 ...
发布时间: 2015 - 05 - 26
浏览次数:205
货号: 13778150  规格  Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells Sample Type (Specific): Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes Product Size: 1.5 mL Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice High Throughput Compatibility: High Throughput-Compatible Green Features: Sustainable packaging  内容及储存  每套包含一管试剂。置于+4°C保存。Lipofectamine® RNAiMAX转染试剂正确储存时可稳定保存1年。  描述  Lipofectamine™ RNAiMAX转染试剂可在多种细胞类型中获得最高的转染效率,适用于siRNA介导的基因抑制实...
发布时间: 2015 - 05 - 26
浏览次数:389
货号: 18064014  规格  Optimal Reaction Temperature: 42° C Final Product Size(s): 12.3 kb or less Reverse Transcriptase: SuperScript® II Ribonuclease H Activity: Reduced Form: Frozen Format: Tube(s) Template: ssDNA, ssRNA, RNA-DNA Hybrid Sensitivity: Medium Product Size: 10,000 units Concentration: 200 U⁄µl Enzyme Function: RNA dependent DNA Polymerase Shipping Condition: Wet Ice Number of Reactions: 50 Reactions  内容及储存  SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl...
发布时间: 2015 - 05 - 26
浏览次数:114
货号: 11765054  规格  Form: Liquid Volume: 500 ml Glutamine: L-Glutamine HEPES Buffer: No HEPES Product Size: 500 mL Phenol Red Indicator: Phenol Red Sodium Pyruvate Additive: Sodium Pyruvate Green Features: Sustainable packaging Shipping Condition: Room Temperature   描述  Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial...
发布时间: 2015 - 05 - 26
浏览次数:125
货号: 12183018A   规格  Sample Type (General): Liquid Samples (e.g. Serum), Bacteria, Yeast, Cells, Plant Samples, Tissue, Blood High Throughput Compatibility: Not High Throughput-Compatible (Manual) Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Next-Generation Sequencing, Nucleic Acid Labeling, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Northern Blotting Format: Spin Column System: PureLink™ Product Size: 50 preps Final Product: Total RN...
发布时间: 2015 - 05 - 26
浏览次数:466
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